In total joint replacement (TJR) the pathogenesis of biomaterial-associated infection (BAI) is dependent on the initial microbial adhesion to biomaterials, a process influenced by microbial characteristics and physico-chemical surface properties of biomaterial: reducing microorganism adhesion to biomaterials could be an attractive means to reduce BAI. In this interdisciplinary study, orthopaedic surgeons, chemists and microbiologists evaluated the influence of Ultra High Molecular Weight Polyethylene (UHMWPE), the most important biomaterial used in TJR,with different surface properties,on the adhesion and subsequent biofilm formation by ATCC and clinical microorganisms by verifying if they could be correlated with lower probability of BAI in TJR patients. We examined the adhesion of two ATCC and one clinical strain of Staphylococcus epidermidis, one ATCC strain of S.aureus and one ATCC strain of Escherichia coli on standard UHMWPE (PE),vitamin E blended polyethylene (VE-PE) and oxidized polyethylene at different incubation times (3,7,24 and 48 hours). Quantitative in vitro analysis of bacterial adhesion was performed by using a sonication protocol to dislodge adherent microorganisms.The biomaterials were physico-chemically characterized by means of scanning electron microscopy, water contact angle measurements and attenuated total reflectance ATR-fourier transform infrared spectroscopy, before and after adhesion assays. Our results indicated that S.epidermidis, S.aureus and E.coli adhesion on UHMWPE is strain dependent, strongly influenced by UHMWPE surface chemistry with a significant decrease in adhered bacteria on VE-PE, compared to that on standard PE: the VE-PE could have the potential to reduce microbial adhesive ability thanks to vitamin E antioxidant properties, regardless of its concentration. The knowledge from this study may have important clinical implications concerning one aspect of the multifactorial septic loosening in TJR,in hopes of pointing out infection resistant biomaterials as advanced technologies: their use in orthopaedic surgery could ensure a significant improvement in patient state of health, with a correlated reduction in welfare costs and an important impact on the socio-economic system.

PROSTHETIC POLYETHYLENES WITH DIFFERENT CHEMICAL AND PHYSICAL PROPERTIES DO MODULATE THE BACTERIAL ADHESION

ALLIZOND, VALERIA;BANCHE, Giuliana;BRACCO, Pierangiola;TULLIO, Viviana Cristina;MANDRAS, Narcisa;BRACH DEL PREVER, Elena Maria;CUFFINI, Annamaria
2012

Abstract

In total joint replacement (TJR) the pathogenesis of biomaterial-associated infection (BAI) is dependent on the initial microbial adhesion to biomaterials, a process influenced by microbial characteristics and physico-chemical surface properties of biomaterial: reducing microorganism adhesion to biomaterials could be an attractive means to reduce BAI. In this interdisciplinary study, orthopaedic surgeons, chemists and microbiologists evaluated the influence of Ultra High Molecular Weight Polyethylene (UHMWPE), the most important biomaterial used in TJR,with different surface properties,on the adhesion and subsequent biofilm formation by ATCC and clinical microorganisms by verifying if they could be correlated with lower probability of BAI in TJR patients. We examined the adhesion of two ATCC and one clinical strain of Staphylococcus epidermidis, one ATCC strain of S.aureus and one ATCC strain of Escherichia coli on standard UHMWPE (PE),vitamin E blended polyethylene (VE-PE) and oxidized polyethylene at different incubation times (3,7,24 and 48 hours). Quantitative in vitro analysis of bacterial adhesion was performed by using a sonication protocol to dislodge adherent microorganisms.The biomaterials were physico-chemically characterized by means of scanning electron microscopy, water contact angle measurements and attenuated total reflectance ATR-fourier transform infrared spectroscopy, before and after adhesion assays. Our results indicated that S.epidermidis, S.aureus and E.coli adhesion on UHMWPE is strain dependent, strongly influenced by UHMWPE surface chemistry with a significant decrease in adhered bacteria on VE-PE, compared to that on standard PE: the VE-PE could have the potential to reduce microbial adhesive ability thanks to vitamin E antioxidant properties, regardless of its concentration. The knowledge from this study may have important clinical implications concerning one aspect of the multifactorial septic loosening in TJR,in hopes of pointing out infection resistant biomaterials as advanced technologies: their use in orthopaedic surgery could ensure a significant improvement in patient state of health, with a correlated reduction in welfare costs and an important impact on the socio-economic system.
40° Congresso Nazionale della Società Italiana di Microbiologia (SIM)
Riccione
7-10 ottobre 2012
Bollettino della SIM
Società Italiana di Microbiologia
XIV (1)
183
183
www.societasim.it
biomaterial-associated infection, UHMWPE, S.epidermidis, S.aureus
V. Allizond; G. Banche; P. Bracco; A. Bistolfi; V. Tullio; M. Boffano; N. Mandras; E. Brach Del Prever; A Cuffini
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/124327
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