BACKGROUND: Human endogenous retrovirus (HERVs) constitute approximately 8% of the human genome. Induction of HERV transcription is possible under certain circumstances, and may have a possible role in some pathological conditions. OBJECTIVES: The aim of this study was to evaluate HERV-K and -W pol gene expression in kidney transplant recipients and to investigate the possible relationship between HERVs gene expression and CMV infection in these patients. STUDY DESIGN: Thirty-three samples of kidney transplant patients and twenty healthy blood donors were used to analyze, HERV-K and -W pol gene RNA expression by relative quantitative relative Real-Time PCR. RESULT: We demonstrated that HERVs pol gene expression levels were higher in kidney transplant recipients than in healthy subjects. Moreover, HERV-K and -W pol gene expression was significantly higher in the group of kidney transplant recipients with high CMV viral load than in the groups with no or moderate CMV viral load. CONCLUSION: Our data suggest that CMV may facilitate in vivo HERV activation.

CMV induces HERV-K and HERV-W expression in kidney transplant recipients

BERGALLO, Massimiliano
First
;
GALLIANO, Ilaria;MONTANARI, Paola;Gambarino, Stefano;MARESCHI, Katia;Fagioli, Franca;TOVO, Pier Angelo;
2015-01-01

Abstract

BACKGROUND: Human endogenous retrovirus (HERVs) constitute approximately 8% of the human genome. Induction of HERV transcription is possible under certain circumstances, and may have a possible role in some pathological conditions. OBJECTIVES: The aim of this study was to evaluate HERV-K and -W pol gene expression in kidney transplant recipients and to investigate the possible relationship between HERVs gene expression and CMV infection in these patients. STUDY DESIGN: Thirty-three samples of kidney transplant patients and twenty healthy blood donors were used to analyze, HERV-K and -W pol gene RNA expression by relative quantitative relative Real-Time PCR. RESULT: We demonstrated that HERVs pol gene expression levels were higher in kidney transplant recipients than in healthy subjects. Moreover, HERV-K and -W pol gene expression was significantly higher in the group of kidney transplant recipients with high CMV viral load than in the groups with no or moderate CMV viral load. CONCLUSION: Our data suggest that CMV may facilitate in vivo HERV activation.
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www.elsevier.com/inca/publications/store/5/2/4/0/6/2
Biomarker; Cancer; Cytomegalovirus; HERV; Retrovirus; Transplant recipient; Virology; Infectious Diseases
Bergallo, Massimiliano; Galliano, Ilaria; Montanari, Paola; Gambarino, Stefano; Mareschi, Katia; Ferro, Francesca; Fagioli, Franca; Tovo, Pier-Angelo; Ravanini, Paolo
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1522063
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