Introduction Exposure to particulate matter (PM) is associated with respiratory and cardiovascular disease and lung cancer. The finest fractions of PM (PM2.5 µm and less) play the major role in causing chronic diseases. The structure and composition of PM influence the biological properties of particles. The aim of the MAPEC study is to evaluate the association between air pollution and early biomarkers in oral mucosa of children recruited from first grade schools of 5 Italian towns characterized by different PM levels. Methods To evaluate children exposure, PM0.5 samples (n=20) were collected (72h) in the school area on the same days of biological samplings (winter 2014-2015) using a high-volume air sampler. PM0.5 organic extracts were chemically analyzed (PAH, Nitro-PAH) and tested on A549 by the comet assay and micronucleus test and on Salmonella strains (TA100, TA98, TA98NR and YG1021) by Ames test. Results Results showed that PM0.5 represents a high variable PM10 percentage (range 19.6-63%). PM10 concentration were generally lower than 50µg/m3 (EU daily limit). All PM0.5 extracts showed a mutagenic effect with TA98 strain (net revertant/m3 range 0.3-1.5) and suggested the presence of indirect mutagens, while lower effect was observed with TA100 strain. The results with the TA98NR and YG1021 strains showed the presence of nitroaromatic compounds as confirmed by the chemical analysis. No genotoxic or oxidative effect of PM0.5 extracts was observed using the comet assay (with/without Fpg enzyme) and micronucleus test except for some sporadic samples. The low biological effect observed could be related to the low level of air pollution observed in this winter sampling associated to a high atmospheric instability. Conclusions For a greater understanding of the relationship between PM size, composition and biological effects the results obtained in this study suggest to investigate the biological effect of the other PM fractions and in particular of the PM0.5-1 fraction.

MUTAGENIC AND GENOTOXIC EFFECT OF PM0.5 IN DIFFERENT ITALIAN TOWNS: THE MAPEC (Monitoring Air Pollution Effects on Children for supporting public health policy) STUDY

BONETTA, Sara;BONETTA, SILVIA;ROMANAZZI, VALERIA;SCHILIRO', Tiziana;PIGNATA, Cristina;GILLI, Giorgio;CARRARO, Elisabetta;
2016

Abstract

Introduction Exposure to particulate matter (PM) is associated with respiratory and cardiovascular disease and lung cancer. The finest fractions of PM (PM2.5 µm and less) play the major role in causing chronic diseases. The structure and composition of PM influence the biological properties of particles. The aim of the MAPEC study is to evaluate the association between air pollution and early biomarkers in oral mucosa of children recruited from first grade schools of 5 Italian towns characterized by different PM levels. Methods To evaluate children exposure, PM0.5 samples (n=20) were collected (72h) in the school area on the same days of biological samplings (winter 2014-2015) using a high-volume air sampler. PM0.5 organic extracts were chemically analyzed (PAH, Nitro-PAH) and tested on A549 by the comet assay and micronucleus test and on Salmonella strains (TA100, TA98, TA98NR and YG1021) by Ames test. Results Results showed that PM0.5 represents a high variable PM10 percentage (range 19.6-63%). PM10 concentration were generally lower than 50µg/m3 (EU daily limit). All PM0.5 extracts showed a mutagenic effect with TA98 strain (net revertant/m3 range 0.3-1.5) and suggested the presence of indirect mutagens, while lower effect was observed with TA100 strain. The results with the TA98NR and YG1021 strains showed the presence of nitroaromatic compounds as confirmed by the chemical analysis. No genotoxic or oxidative effect of PM0.5 extracts was observed using the comet assay (with/without Fpg enzyme) and micronucleus test except for some sporadic samples. The low biological effect observed could be related to the low level of air pollution observed in this winter sampling associated to a high atmospheric instability. Conclusions For a greater understanding of the relationship between PM size, composition and biological effects the results obtained in this study suggest to investigate the biological effect of the other PM fractions and in particular of the PM0.5-1 fraction.
28° Conference of the International Society for Environmental Epidemiology
Roma
1-4 settembre 2016
Abstract book - 28° Conference of the International Society for Environmental Epidemiology 28th Annual Conference International Society for Environmental Epidemiology - Old and new risks: challenges for environmental epidemiology 1-4 September 2016 / Rome
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particulate matter, genotoxicity, comet assay, Ames test, Micronucleus test, A549
Bonetta, Sa.; Bonetta, Si; Ceretti, E.; Viola, G.C.V.; Feretti, D.; Romanazzi, V.; Levorato, S.; Salvatori, T.; Vannini, S.; Schilirò, T.; Verani, M.; Pignata, C.; Serio, F.; Gilli, G.; Bonizzoni, S.; Bonetti, A.; Carraro, E.; Gelatti, U.; Mapec_life, Study Group.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/1621235
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