1. Introduction Mixtures of molecules derived from plant natural extracts (egg. polyphenols, essential oils) have interesting properties as antibacterial, antitumoral, antinflammatory and osteoinductive activity. It is possible to combine the properties of these molecules with those of bioactive materials used in bone contact application by mean of surface functionalization. As an alternative polyphenols are also reductive agents useful for the in situ reduction of silver nanoparticles in order to confer to the materials inorganic antibacterial activities. 2. Methods The substrates used for the surface functionalization were a silica based bioactive glass and a Ti6Al4V alloy made bioactive by means of a patented chemical treatment. The surfaces were functionalized with different molecules reported in figure 1 (gallic acid as model molecules, polyphenols from red grape skins, green tea leaves and Mentha piperita essential oil of Pancalieri). The functionalization protocols were tailored using different solvents and different time of functionalization suitable for each kind of biomolecules and for the two different substrates. The presence and the activity of the molecules and ions on the surfaces were analyzed by means of XPS, FTIR, gas chromatograpyc and spectroscopic analyses. The effect of these molecules on cells and bacteria was also investigated with biological tests. The silver ions release was investigated in different media. 3. Results The presence and the activity of the polyphenols on the surfaces were proved by means of XPS, FTIR and spectroscopic measurements with the Folin&Ciocalteu methods. Cell cultures also prove the ability of polyphenols to promote osteointegration with healthy osteoblast and induce differentiation in mesenchymal stem cells and promote apoptosis of cancer cells. The presence of the molecules of Mentha oil and silver nanoparticles was checked with GC, FTIR and XPS; they have effect against bacteria, but they show also toxicity against cells. For the samples with silver nanoparticles a well dispersion of the nanoparticles on the substrate and a release at 14 days were observed. 4. Discussion & Conclusion Regarding polyphenols, the surface functionalization seems to be a promising way for in loco use of these molecules rich of beneficial effects on human health. For those concern the samples with the Mentha oil and the silver nanoparticles, the protocol of functionalization needs to be improved in order to tailor the amount of Mentha oil molecules and silver nanoparticles to avoid cytotoxic effects maintaining the great antibacterial activity. The biomaterial surface functionalization is a promising interest field in order to combine the properties of biomolecules with those of biomaterials. It’s possible to confer them new properties using natural molecules with high value but low price that could be also used as additives during different process like nanoparticles reduction.

Plant derived natural molecules: a promising way for surface functionalization of biomaterials in bone contact applications

Cazzola M;Prenesti E;Allizond V;Banche G;Cuffini AM;Bertea CM;
2017

Abstract

1. Introduction Mixtures of molecules derived from plant natural extracts (egg. polyphenols, essential oils) have interesting properties as antibacterial, antitumoral, antinflammatory and osteoinductive activity. It is possible to combine the properties of these molecules with those of bioactive materials used in bone contact application by mean of surface functionalization. As an alternative polyphenols are also reductive agents useful for the in situ reduction of silver nanoparticles in order to confer to the materials inorganic antibacterial activities. 2. Methods The substrates used for the surface functionalization were a silica based bioactive glass and a Ti6Al4V alloy made bioactive by means of a patented chemical treatment. The surfaces were functionalized with different molecules reported in figure 1 (gallic acid as model molecules, polyphenols from red grape skins, green tea leaves and Mentha piperita essential oil of Pancalieri). The functionalization protocols were tailored using different solvents and different time of functionalization suitable for each kind of biomolecules and for the two different substrates. The presence and the activity of the molecules and ions on the surfaces were analyzed by means of XPS, FTIR, gas chromatograpyc and spectroscopic analyses. The effect of these molecules on cells and bacteria was also investigated with biological tests. The silver ions release was investigated in different media. 3. Results The presence and the activity of the polyphenols on the surfaces were proved by means of XPS, FTIR and spectroscopic measurements with the Folin&Ciocalteu methods. Cell cultures also prove the ability of polyphenols to promote osteointegration with healthy osteoblast and induce differentiation in mesenchymal stem cells and promote apoptosis of cancer cells. The presence of the molecules of Mentha oil and silver nanoparticles was checked with GC, FTIR and XPS; they have effect against bacteria, but they show also toxicity against cells. For the samples with silver nanoparticles a well dispersion of the nanoparticles on the substrate and a release at 14 days were observed. 4. Discussion & Conclusion Regarding polyphenols, the surface functionalization seems to be a promising way for in loco use of these molecules rich of beneficial effects on human health. For those concern the samples with the Mentha oil and the silver nanoparticles, the protocol of functionalization needs to be improved in order to tailor the amount of Mentha oil molecules and silver nanoparticles to avoid cytotoxic effects maintaining the great antibacterial activity. The biomaterial surface functionalization is a promising interest field in order to combine the properties of biomolecules with those of biomaterials. It’s possible to confer them new properties using natural molecules with high value but low price that could be also used as additives during different process like nanoparticles reduction.
29th Conference of the Society for Medical Innovation and Technology iSMIT
Torino
9-10 november 2017
Abstract per sesssions
Society for Medical Innovation and Technology iSMIT
182 (O24)
183 (O24)
http://smit2017.com/
Cazzola M, Ferraris S, Cempura G, Prenesti E, Allizond V, Banche G, Cuffini AM, Bertea CM, C A, Rimondini L, Pezzotti G, Spriano S, Vernè E
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/1661019
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