Particulate matter (PM) can be considered as the atmospheric pollutant that mostly affects human health. The International Agency for Research on Cancer (IARC) has recently classified air pollution and fine PM as carcinogenic to human (1 group). Different studies showed that the biological effects induced by PM can vary depending on the cell lines used. The aim of this study was to evaluate the genotoxicity induced byPM0.5 samples comparing the results obtained withtwodifferent cell lines: A549 and BEAS-2B. In the MAPEC LIFE study the PM0.5 samples (n = 36) were collected in 5 Italian towns characterized by different PM levels. PM0.5 organic extracts were chemically analyzed (PAHs, Nitro-PAHs) and tested on A549 and BEAS-2B by the comet assay. Results showed that PM0.5 represents a high variable PM10 percentage (range 20–63%). The highest concentrations of PAHs and Nitro-PAHs were observed in Torino and Brescia. No genotoxic effect of PM0.5 extracts was observed using A549 cells except for one sample. BEAS-2B cells highlighted a light DNA damage of samples collected in Torino, Brescia and Pisa although only at the highest doses tested. The low biological effect observed could be related to the low level of air pollution observed, associated to a high atmospheric instability. Results obtained highlighted the higher sensitivity of BEAS-2B cells respect to A549 also in samples with low level of pollutants, confirming that PM can induce genotoxicity in normal cells while cancer cells can be resistant to its adverse effect.

DNA damage induced by PM0.5 samples in A549 and BEAS-2B human cell lines: Results of the MAPEC study / Bonetta Si., Bonetta Sa., Feretti D., Moretti M., Verani M., De Donno A., Schilirò T., Carraro E., Gelatti U., MAPEC LIFE Study Group.. - In: TOXICOLOGY LETTERS. - ISSN 0378-4274. - 280:1(2017), pp. 208-208. ((Intervento presentato al convegno 53rd Congress of the European Societies of Toxicology (EUROTOX) tenutosi a Bratislava nel 10-13 September.

DNA damage induced by PM0.5 samples in A549 and BEAS-2B human cell lines: Results of the MAPEC study

Bonetta Si.;Bonetta Sa.;Schilirò T.;Carraro E.;
2017

Abstract

Particulate matter (PM) can be considered as the atmospheric pollutant that mostly affects human health. The International Agency for Research on Cancer (IARC) has recently classified air pollution and fine PM as carcinogenic to human (1 group). Different studies showed that the biological effects induced by PM can vary depending on the cell lines used. The aim of this study was to evaluate the genotoxicity induced byPM0.5 samples comparing the results obtained withtwodifferent cell lines: A549 and BEAS-2B. In the MAPEC LIFE study the PM0.5 samples (n = 36) were collected in 5 Italian towns characterized by different PM levels. PM0.5 organic extracts were chemically analyzed (PAHs, Nitro-PAHs) and tested on A549 and BEAS-2B by the comet assay. Results showed that PM0.5 represents a high variable PM10 percentage (range 20–63%). The highest concentrations of PAHs and Nitro-PAHs were observed in Torino and Brescia. No genotoxic effect of PM0.5 extracts was observed using A549 cells except for one sample. BEAS-2B cells highlighted a light DNA damage of samples collected in Torino, Brescia and Pisa although only at the highest doses tested. The low biological effect observed could be related to the low level of air pollution observed, associated to a high atmospheric instability. Results obtained highlighted the higher sensitivity of BEAS-2B cells respect to A549 also in samples with low level of pollutants, confirming that PM can induce genotoxicity in normal cells while cancer cells can be resistant to its adverse effect.
53rd Congress of the European Societies of Toxicology (EUROTOX)
Bratislava
10-13 September
280
1
208
208
Bonetta Si., Bonetta Sa., Feretti D., Moretti M., Verani M., De Donno A., Schilirò T., Carraro E., Gelatti U., MAPEC LIFE Study Group.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/1661706
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