Different studies on titanium dioxide nanoparticles (TiO2-NPs) genotoxicity have been conducted but no clear accordance exists among mechanism of action. Many physico-chemical properties of TiO2-NPs (i.e. shape, crystal structure, aggregation and coatings) can influence biological effects. The aim of this study was to evaluate the genotoxic effect of three engineered TiO2-NPs shapes (bipyramids, rods and platelet NPs) in bronchial epithelial cells (BEAS-2B) in comparison with two commercial TiO2-NPs (p25 and food grade). Detailed characteristics of TiO2-NPs dispersions were defined (T-SEM, DLS by SETNanoMetro project). BEAS-2B were exposed to different doses of TiO2-NPs (range 0 - 120 µg/ml) for 24h (1h light, 23h dark). Genotoxic and oxidative damage was assessed by Comet assay (with/without Fpg enzyme). Cytotoxic effects were also evaluated by cell viability assays (WST-1) and membrane damage (LDH assay). Moderate viability reduction (88-96%, p<0.05) was detected in BEAS-2B exposed to the highest concentration of all TiO2-NPs. No significant membrane damage was observed confirming the low cytotoxic effect. A significant (p<0.05) dose-response DNA damage (direct and oxidative) was induced by food grade NPs, while p25 showed only oxidative damage. Bipyramids and rods TiO2-NPs did not show any genotoxic effect; platelet TiO2-NPs induced direct and oxidative DNA damage at the highest doses (p<0.05) and it could be probably related to the higher aggregation tendency. The commercial TiO2-NPs had a higher genotoxic effect than shape engineered ones, however the shape induced different genotoxic effects. This study suggests higher safety in using shape engineered TiO2-NPs for different technological applications (i.e. paints, foods, cosmetics, pharmaceuticals).

Genotoxic and oxidative damage of shape-ebgineered Titanium dioxide nanoparticoles (TiO2-NPs) in bronchial epithelial cells. / Bonetta Sa; Gea M.; Bonetta Si; Innarelli L.; Rossi A.M.; Maurino V.; Gilli G.; Schilirò T.. - In: MUTAGENESIS. - ISSN 0267-8357. - 32:6(2017), pp. 13-13. ((Intervento presentato al convegno 12° International Comet Assay Workshop (ICAW) tenutosi a Pamplona nel 29-31 August.

Genotoxic and oxidative damage of shape-ebgineered Titanium dioxide nanoparticoles (TiO2-NPs) in bronchial epithelial cells.

Bonetta Sa;Gea M.;Bonetta Si;Maurino V.;Gilli G.;Schilirò T.
2017

Abstract

Different studies on titanium dioxide nanoparticles (TiO2-NPs) genotoxicity have been conducted but no clear accordance exists among mechanism of action. Many physico-chemical properties of TiO2-NPs (i.e. shape, crystal structure, aggregation and coatings) can influence biological effects. The aim of this study was to evaluate the genotoxic effect of three engineered TiO2-NPs shapes (bipyramids, rods and platelet NPs) in bronchial epithelial cells (BEAS-2B) in comparison with two commercial TiO2-NPs (p25 and food grade). Detailed characteristics of TiO2-NPs dispersions were defined (T-SEM, DLS by SETNanoMetro project). BEAS-2B were exposed to different doses of TiO2-NPs (range 0 - 120 µg/ml) for 24h (1h light, 23h dark). Genotoxic and oxidative damage was assessed by Comet assay (with/without Fpg enzyme). Cytotoxic effects were also evaluated by cell viability assays (WST-1) and membrane damage (LDH assay). Moderate viability reduction (88-96%, p<0.05) was detected in BEAS-2B exposed to the highest concentration of all TiO2-NPs. No significant membrane damage was observed confirming the low cytotoxic effect. A significant (p<0.05) dose-response DNA damage (direct and oxidative) was induced by food grade NPs, while p25 showed only oxidative damage. Bipyramids and rods TiO2-NPs did not show any genotoxic effect; platelet TiO2-NPs induced direct and oxidative DNA damage at the highest doses (p<0.05) and it could be probably related to the higher aggregation tendency. The commercial TiO2-NPs had a higher genotoxic effect than shape engineered ones, however the shape induced different genotoxic effects. This study suggests higher safety in using shape engineered TiO2-NPs for different technological applications (i.e. paints, foods, cosmetics, pharmaceuticals).
12° International Comet Assay Workshop (ICAW)
Pamplona
29-31 August
32
6
13
13
TiO2, nanoparticles, genotoxicity, Comet assay
Bonetta Sa; Gea M.; Bonetta Si; Innarelli L.; Rossi A.M.; Maurino V.; Gilli G.; Schilirò T.
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/1664473
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