The apolipoprotein B editing enzyme catalytic subunit 3 (APOBEC3) is a family of DNA cytosine deaminases that mutate and inactivate viral genomes by single-strand DNA editing, thus providing an innate immune response against a wide range of DNA and RNA viruses. In particular, APOBEC3A (A3A), a member of the APOBEC3 family, is induced by human cytomegalovirus (HCMV) in decidual tissues where it efficiently restricts HCMV replication, thereby acting as an intrinsic innate immune effector at the maternal-fetal interface. However, the widespread incidence of congenital HCMV infection implies that HCMV has evolved to counteract APOBEC3-induced mutagenesis through mechanisms that still remain to be fully established. Here, we have assessed gene expression and deaminase activity of various APOBEC3 gene family members in HCMV-infected primary human foreskin fibroblasts (HFFs). Specifically, we show that APOBEC3G (A3G) and to a lesser degree A3F, but not A3A, gene products are upregulated in HCMV-infected HFFs. We also show that HCMV mediated induction of A3G expression is mediated by interferon-β (IFN-β), which is produced early during HCMV infection. However, knockdown or overexpression of A3G does not affect HCMV replication, indicating that A3G is not a restriction factor for HCMV. Finally, through a bioinformatics approach, we show that HCMV has evolved mutational robustness against IFN-β by limiting the presence of A3G hotspots in essential open reading frames (ORFs) of its genome. Overall, our findings uncover a novel immune evasion strategy by HCMV with profound implications for HCMV infections

Evasion strategy of Human Cytomegalovirus from the antiviral activities of APOBEC3G

Matteo Biolatti;Sara Pautasso;Ganna Galitska;Valentina Dell Oste;Marco De Andrea;Marisa Gariglio;Santo Landolfo
2018

Abstract

The apolipoprotein B editing enzyme catalytic subunit 3 (APOBEC3) is a family of DNA cytosine deaminases that mutate and inactivate viral genomes by single-strand DNA editing, thus providing an innate immune response against a wide range of DNA and RNA viruses. In particular, APOBEC3A (A3A), a member of the APOBEC3 family, is induced by human cytomegalovirus (HCMV) in decidual tissues where it efficiently restricts HCMV replication, thereby acting as an intrinsic innate immune effector at the maternal-fetal interface. However, the widespread incidence of congenital HCMV infection implies that HCMV has evolved to counteract APOBEC3-induced mutagenesis through mechanisms that still remain to be fully established. Here, we have assessed gene expression and deaminase activity of various APOBEC3 gene family members in HCMV-infected primary human foreskin fibroblasts (HFFs). Specifically, we show that APOBEC3G (A3G) and to a lesser degree A3F, but not A3A, gene products are upregulated in HCMV-infected HFFs. We also show that HCMV mediated induction of A3G expression is mediated by interferon-β (IFN-β), which is produced early during HCMV infection. However, knockdown or overexpression of A3G does not affect HCMV replication, indicating that A3G is not a restriction factor for HCMV. Finally, through a bioinformatics approach, we show that HCMV has evolved mutational robustness against IFN-β by limiting the presence of A3G hotspots in essential open reading frames (ORFs) of its genome. Overall, our findings uncover a novel immune evasion strategy by HCMV with profound implications for HCMV infections
4th Innovative Approaches for Identification of Antiviral Agents Summer School
Santa Margherita di Pula, Sardinia, Italy
24-28 settembre
4th Innovative Approaches for Identification of Antiviral Agents Summer School
28
28
Matteo Biolatti, Sara Pautasso, Ganna Galitska, Valentina Dell Oste, Rachele Cagliani, Diego Forni, Marco De Andrea, Marisa Gariglio, Manuela Sironi, Santo Landolfo
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/1677876
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