The present study analyzes the effect of murine IFN-gamma on DNA synthesis, ornithine decarboxylase activity and phosphorylation of pp60src of Rouse sarcoma virus transformed cells. When natural or recombinant IFN-gamma was added to quiescent SR-BALB or L1210 cells, stimulated with fetal calf serum, only the highest IFN-gamma concentrations (2000 U/ml) inhibited DNA synthesis of both tumor lines. By contrast, lower IFN-concentrations (20-200 U/ml) inhibited the DNA synthesis of L1210 but not of SR-BALB tumor cells. A similar pattern of inhibition was observed when ornithine decarboxylase activity was analyzed. Finally, when the levels of phosphorylation in SR-BALB cells were analyzed after IFN-gamma treatment, no difference with untreated controls was observed, even at the highest concentrations. These results suggest that SR-BALB tumor cells are insensitive to IFN-gamma and that src oncogene activity is not affected at IFN-concentrations inhibiting cell growth.

Interferon-gamma inhibits cell replication, but not pp60src activity of RSV-transformed fibroblasts. / GRIBAUDO G ;DI RENZO F ;NALDINI L ;COMOGLIO PM ;CAVALLO G ;LANDOLFO S. - In: MICROBIOLOGICA. - ISSN 0391-5352. - 10(1987), pp. 47-53.

Interferon-gamma inhibits cell replication, but not pp60src activity of RSV-transformed fibroblasts.

GRIBAUDO, Giorgio;DI RENZO, Maria Flavia;COMOGLIO, Paolo;CAVALLO, Giorgio;LANDOLFO, Santo Giuseppe
1987

Abstract

The present study analyzes the effect of murine IFN-gamma on DNA synthesis, ornithine decarboxylase activity and phosphorylation of pp60src of Rouse sarcoma virus transformed cells. When natural or recombinant IFN-gamma was added to quiescent SR-BALB or L1210 cells, stimulated with fetal calf serum, only the highest IFN-gamma concentrations (2000 U/ml) inhibited DNA synthesis of both tumor lines. By contrast, lower IFN-concentrations (20-200 U/ml) inhibited the DNA synthesis of L1210 but not of SR-BALB tumor cells. A similar pattern of inhibition was observed when ornithine decarboxylase activity was analyzed. Finally, when the levels of phosphorylation in SR-BALB cells were analyzed after IFN-gamma treatment, no difference with untreated controls was observed, even at the highest concentrations. These results suggest that SR-BALB tumor cells are insensitive to IFN-gamma and that src oncogene activity is not affected at IFN-concentrations inhibiting cell growth.
10
47
53
GRIBAUDO G ;DI RENZO F ;NALDINI L ;COMOGLIO PM ;CAVALLO G ;LANDOLFO S
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/29791
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