Rubella virus hemagglutination non-specific inhibitors, in sera fractionated on Bio-Gel A 5 m, elute in IgM containing fractions. In addition, in heparin-MnCl2 treated sera, the same inhibitors display a different chromatographic behaviour on Bio-Gel A 5m, being eluted earlier than IgM. Therefore, in order to avoid false-positive results due to the presence of non-specific inhibitors in IgM containing fractions it is necessary to verify the disappearance of hemagglutination-inhibition activity after 2-ME reduction.

[Fractionation of serum by gel filtration: contamination of IgM fractions by non-specific inhibitors of hemagglutination of the rubella virus] / MERLINO C ;ANGERETTI A ;NEGRO PONZI A. - In: GIORNALE DI BATTERIOLOGIA, VIROLOGIA ED IMMUNOLOGIA. - ISSN 0390-5462. - 76(1983), pp. 109-117.

[Fractionation of serum by gel filtration: contamination of IgM fractions by non-specific inhibitors of hemagglutination of the rubella virus]

MERLINO, Chiara;
1983

Abstract

Rubella virus hemagglutination non-specific inhibitors, in sera fractionated on Bio-Gel A 5 m, elute in IgM containing fractions. In addition, in heparin-MnCl2 treated sera, the same inhibitors display a different chromatographic behaviour on Bio-Gel A 5m, being eluted earlier than IgM. Therefore, in order to avoid false-positive results due to the presence of non-specific inhibitors in IgM containing fractions it is necessary to verify the disappearance of hemagglutination-inhibition activity after 2-ME reduction.
76
109
117
MERLINO C ;ANGERETTI A ;NEGRO PONZI A
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/30982
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