Variants of parvovirus B19 are currently grouped into three genotypes: 1 (reference B19 strains), 2 and 3. It has been evidenced that isolate K71 of genotype 2 is more prevalent in skin than the conventional B19 genotype 1. In this study we investigated the detection of parvovirus B19 genotypes by using two nested PCRs and evaluating the suitability of these assays by BLAST search of parvovirus isolates. Subsequently, we analyze the present genotypes in skin biopsies. The two nested PCRs employed in this study allow to amplify 41 isolates as confirmed by bioinformatical validation. The molecular epidemiological characterization of our casistics confirmed the presence of isolate K71 in human skin.

Variants of parvovirus B19: Bioinformatical evaluation of nested PCR assays and gentoypes detection

BERGALLO, Massimiliano;Costa C.;SIDOTI, Francesca;NOVELLI, Mauro;MERLINO, Chiara;BERNENGO, Maria Grazia;CAVALLO, Rossana
2008

Abstract

Variants of parvovirus B19 are currently grouped into three genotypes: 1 (reference B19 strains), 2 and 3. It has been evidenced that isolate K71 of genotype 2 is more prevalent in skin than the conventional B19 genotype 1. In this study we investigated the detection of parvovirus B19 genotypes by using two nested PCRs and evaluating the suitability of these assays by BLAST search of parvovirus isolates. Subsequently, we analyze the present genotypes in skin biopsies. The two nested PCRs employed in this study allow to amplify 41 isolates as confirmed by bioinformatical validation. The molecular epidemiological characterization of our casistics confirmed the presence of isolate K71 in human skin.
51
2
75
80
http://www.karger.com/Article/FullText/127429
Parvovirus B19; Nested PCR; Human Skin
Bergallo M.; Costa C.; Sidoti F.; Novelli M.; Ponti R.; Castagnoli C.; Merlino C.; Bernengo M.G.; Cavallo R.
File in questo prodotto:
File Dimensione Formato  
Parvovirus B19 2007.pdf

Accesso riservato

Tipo di file: PDF EDITORIALE
Dimensione 170.44 kB
Formato Adobe PDF
170.44 kB Adobe PDF   Visualizza/Apri   Richiedi una copia

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/68909
Citazioni
  • ???jsp.display-item.citation.pmc??? 2
  • Scopus 12
  • ???jsp.display-item.citation.isi??? 10
social impact