Aims: To compare the efficiency of the ScanVIT-LegionellaTM test (Vermicon,Munich, Germany) vs a conventional culture method for the quantification of Legionella spp. in hospital water samples in daily hospital practice. Methods and Results: The detection of Legionella spp. takes place on a cultivated filter brought into contact with dye-marked gene probes. The results are analysed under fluorescence microscopy. Bacteria that light up green belong to the genus Legionella; those that light up both green and red belong to the species Legionella pneumophila. Our results showed that the ScanVIT test has a sensitivity of 90%; agreement between the two methods was 82%. In the 48 samples that tested positive with both methods, the Legionella concentration detected by the culture method was consistently higher. A statistically significant difference between the results obtained with the two test methods emerged at the Wilcoxon test (P < 0Æ001). Conclusion: The ScanVIT test may be recommended for investigating the presence of Legionella by qualitative testing. Significance and Impact of the Study: Given the simplicity of colony identification by fluorescence, the ScanVIT test can be used in laboratories where staffs are not experienced in identifying typical colonies of Legionella.

Evaluation of the usefulness of a new direct immunofluorescence assay (ScanVIT-Legionella) for monitoring hospital water systems contaminated with Legionella spp

DITOMMASO, Savina;GIACOMUZZI, Monica;ZOTTI, Carla Maria
2010-01-01

Abstract

Aims: To compare the efficiency of the ScanVIT-LegionellaTM test (Vermicon,Munich, Germany) vs a conventional culture method for the quantification of Legionella spp. in hospital water samples in daily hospital practice. Methods and Results: The detection of Legionella spp. takes place on a cultivated filter brought into contact with dye-marked gene probes. The results are analysed under fluorescence microscopy. Bacteria that light up green belong to the genus Legionella; those that light up both green and red belong to the species Legionella pneumophila. Our results showed that the ScanVIT test has a sensitivity of 90%; agreement between the two methods was 82%. In the 48 samples that tested positive with both methods, the Legionella concentration detected by the culture method was consistently higher. A statistically significant difference between the results obtained with the two test methods emerged at the Wilcoxon test (P < 0Æ001). Conclusion: The ScanVIT test may be recommended for investigating the presence of Legionella by qualitative testing. Significance and Impact of the Study: Given the simplicity of colony identification by fluorescence, the ScanVIT test can be used in laboratories where staffs are not experienced in identifying typical colonies of Legionella.
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http://onlinelibrary.wiley.com/doi/10.1111/j.1472-765X.2010.02797.x/abstract;jsessionid=6B811D4649DB3A4695FBBC222F58647B.f02t01
environmental water samples; immunofluorescence Legionella quantification standard culture method
Ditommaso S; Giacomuzzi M; Gentile M; Zotti CM.
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2318/73532
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