Methanogen populations and their domains are poorly understood; however, in recent years, research on this topic has emerged. The relevance of this field has also been enhanced by the growing economic interest in methanogen skills, particularly the production of methane from organic substrates. Management attention turned to anaerobic wastes digestion because the volume and environmental impact reductions. Methanogenesis is the biochemically limiting step of the process and the industrially interesting phase because it connects to the amount of biogas production. For this reason, several studies have evaluated the structure of methanogen communities during this process. Currently, it is clear that the methanogen load and diversity depend on the feeding characteristics and the process conditions, but not much data is available. In this study, we apply a Real-Time Polymerase Chain Reaction (RT-PCR) method based on mcrA target to evaluate, by specific probes, some subgroups of methanogens during the mesophilic anaerobic digestion process fed wastewater sludge and organic fraction of the municipal solid waste with two different pre-treatments. The obtained data showed the prevalence of Methanomicrobiales and significantly positive correlation between Methanosarcina and Methanosaetae and the biogas production rate (0.744 p<0.01 and 0.641 p<0.05). Methanosarcina detected levels are different during the process after the two pre-treatment of the input materials (T-test p<0.05). Moreover, a role as diagnostic tool could be suggested in digestion optimisation.

The role of different methanogen groups evaluated by Real-Time qPCR as high-efficiency bioindicators of wet anaerobic co-digestion of organic waste

TRAVERSI, Deborah;DEGAN, Raffaella;GILLI, Giorgio
2011

Abstract

Methanogen populations and their domains are poorly understood; however, in recent years, research on this topic has emerged. The relevance of this field has also been enhanced by the growing economic interest in methanogen skills, particularly the production of methane from organic substrates. Management attention turned to anaerobic wastes digestion because the volume and environmental impact reductions. Methanogenesis is the biochemically limiting step of the process and the industrially interesting phase because it connects to the amount of biogas production. For this reason, several studies have evaluated the structure of methanogen communities during this process. Currently, it is clear that the methanogen load and diversity depend on the feeding characteristics and the process conditions, but not much data is available. In this study, we apply a Real-Time Polymerase Chain Reaction (RT-PCR) method based on mcrA target to evaluate, by specific probes, some subgroups of methanogens during the mesophilic anaerobic digestion process fed wastewater sludge and organic fraction of the municipal solid waste with two different pre-treatments. The obtained data showed the prevalence of Methanomicrobiales and significantly positive correlation between Methanosarcina and Methanosaetae and the biogas production rate (0.744 p<0.01 and 0.641 p<0.05). Methanosarcina detected levels are different during the process after the two pre-treatment of the input materials (T-test p<0.05). Moreover, a role as diagnostic tool could be suggested in digestion optimisation.
1
1
28
methanogen; anaerobic digestion; biogas production; Methanosarcina; Archaea communities
Deborah Traversi; Silvia Villa; Marco Acri; Biancamaria Pietrangeli; Raffaella Degan; Giorgio Gilli
File in questo prodotto:
File Dimensione Formato  
Traversi, AMB2011.pdf

Accesso aperto

Tipo di file: PDF EDITORIALE
Dimensione 325.59 kB
Formato Adobe PDF
325.59 kB Adobe PDF Visualizza/Apri

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2318/92871
Citazioni
  • ???jsp.display-item.citation.pmc??? 5
  • Scopus 30
  • ???jsp.display-item.citation.isi??? 17
social impact